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However, the expression of COXs and LOXs in astrocytes is still largely unknown. In the present study, we investigated the regulatory mechanism of prostaglandin synthesis in cultured human brain astrocytes. The astrocytes express COX-1, COX-2, LOX-5, and 15-LOX-1 mRNA. The COX-2 protein is constitutively expressed in astrocytes. Prostaglandin E2, but not prostaglandin F2alpha, has been found to stimulate the proliferation of astrocytes in a concentration-dependent manner. Cyclooxygenase inhibition by indomethacin or diclofenac reduces prostaglandin E2-induced proliferation. 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